Holý L, Vanatka F, Lopatárová M
Vysoká skola veterinární, Brno.
Vet Med (Praha). 1992 Jul;37(7):353-64.
The descent and localization of eggs and embryos in individual segments of the reproductive tract of superovulated cows were studied in this work. For the induction of superovulation, serum gonadotropin (PMSG, Ivanovice in Haná) at a dose of 2,500-3,500 I.U. was used, in combination with 0.5 mg of Cloprostenol (Oestrophan, Spofa), administered 48 hours after gonadotropin treatment. The start of superovulation fell on days 9 to 12 of the sexual cycle and was conditioned by the presence of the corpus luteum (CL). After the onset of the heat, 2-3 inseminations were carried out using fresh semen. Donor cows were slaughtered 3, 4, 5, 6, and 7 days after the second insemination and isolated reproductive organs (Fig. 1) were divided into five segments (two on oviducts and three on uterine horns) by the applied ligature. In laboratory conditions superovulation response was determined accurately, the volume of ovaries was assessed according to water displacement and the segments of oviducts and uterus were rinsed with TCM 199 or PBS supplemented with FCS. 3, 4, 5, 6, and 7 days after insemination (Tab. I). 18.1 (+/- 3.55), 12.4 (+/- 0.91), 19.2 (+/- 2.86), 20 and 23 (+/- 2.44) CL on average were recorded, which corresponded to the ovulation of 64, 50, 56, 71 and 72 percent of stimulated follicles (Fig. 2). Within 3 to 7 days after insemination nearly triple enlargement of ovaries was also observed (Tab. I, Fig. 3). During the lavage of individual segments of the tubular reproductive tract, 38 per cent of eggs and embryos were detected in the uterus as early as 3 days after insemination (Tab. II). Unfertilized eggs and degenerated embryos were found in the 2nd and 3rd uterine segment, embryos at the stage of 8-16 blastomeres were localized in the 1st and 2nd segment of the uterus. Four days after insemination (Tab. III), about 64 per cent of eggs and embryos at the stage up to 16 blastomeres were found in the uterus, but embryos up to 32 blastomeres were still flushed out of the oviduct. On day 5 after insemination, 92 per cent of eggs and embryos were released into the uterus, being localized mostly in the cranial and medial part of the uterus (Tab. IV). 7.5 per cent of recovered eggs and embryos at the stage of early or compacted morulae were still detected in the oviducts.(ABSTRACT TRUNCATED AT 400 WORDS)
本研究对超排奶牛生殖道各段中卵子和胚胎的下行及定位情况进行了研究。为诱导超排，使用剂量为2500 - 3500国际单位的血清促性腺激素（孕马血清促性腺激素，汉纳的伊凡诺维采），并在促性腺激素处理48小时后联合使用0.5毫克氯前列醇（奥施康定，斯波法）。超排开始于性周期的第9至12天，以黄体的存在为条件。发情开始后，使用新鲜精液进行2 - 3次输精。在第二次输精后3、4、5、6和7天屠宰供体奶牛，将分离出的生殖器官（图1）通过结扎分为五个部分（输卵管两个部分，子宫角三个部分）。在实验室条件下准确测定超排反应，根据排水法评估卵巢体积，并用添加了胎牛血清的TCM 199或PBS冲洗输卵管和子宫的各部分。输精后3、4、5、6和7天（表I）。平均记录到18.1（±3.55）、12.4（±0.91）、19.2（±2.86）、20和23（±2.44）个黄体，分别对应64%、50%、56%、71%和72%的刺激卵泡排卵（图2）。在输精后3至7天内还观察到卵巢几乎增大了两倍（表I，图3）。在冲洗管状生殖道的各个部分时，早在输精后3天就在子宫中检测到38%的卵子和胚胎（表II）。在子宫的第二和第三部分发现未受精卵和退化胚胎，8 - 16细胞期的胚胎位于子宫的第一和第二部分。输精后4天（表III），在子宫中发现约64%的卵子和16细胞期之前的胚胎，但32细胞期之前的胚胎仍从输卵管中冲洗出来。输精后第5天，92%的卵子和胚胎进入子宫，主要位于子宫的头侧和内侧部分（表IV）。在输卵管中仍检测到7.5%处于早期或致密桑葚胚阶段的回收卵子和胚胎。（摘要截于400字）
