Betteridge K J, Eaglesome M D, Randall G C, Mitchell D
J Reprod Fertil. 1980 May;59(1):205-16. doi: 10.1530/jrf.0.0590205.
Of 226 donor cattle treated with PMSG to induce superovulation, 76.5% responded with 3 or more ovulations. Flushing at surgery or slaughter 10-16 days after oestrus recovered eggs and embryos that represented 49.3% of the number of ovulations. Of those recovered, 73.3% were embryos, an average yield of 4.0 embryos/treated cow or 4.8 embryo/flushed cow. The location of eggs and embryos was determined in 65 of the donors. Embryos and unfertilized eggs (6.1% of those recovered) were occasionally found in the oviducts. Empty zonae pellucidae were also found in the uterus on all days. The lengths, or diameters, of embryos were extremely variable within days and within donors, but mean values indicated logarithmic growth between Days 10 and 16. Eighty-four synchronous (+/- 1 day) recipients received single embryos, and 51 recipients twin embryos, by surgical transfer. Pregnancies were obtained in recipients up to Day 16 but not on Day 17, indicating the stage by which an embryo must be present to prevent luteolysis. The overall pregnancy rate at Day 42 was 50.4% and further 18.1% of the recipients exhibited extended oestrous cycles. Of 35 recipients that were allowed to go to term, 12 lost their pregnancies, most often between Days 42 and 63.
对226头供体牛使用孕马血清促性腺激素(PMSG)诱导超排卵,76.5%的牛出现3次或更多次排卵。在发情后10 - 16天进行手术或屠宰时冲洗,回收的卵子和胚胎数量占排卵数的49.3%。回收的胚胎中,73.3%为胚胎,平均每头处理过的母牛回收4.0个胚胎,或每头冲洗过的母牛回收4.8个胚胎。在65头供体牛中确定了卵子和胚胎的位置。胚胎和未受精卵(占回收数量的6.1%)偶尔在输卵管中发现。在所有天数中子宫内也发现了空透明带。胚胎在不同天数和不同供体之间的长度或直径变化极大,但平均值表明在第10天到第16天之间呈对数生长。84头同步(±1天)受体通过手术移植接受单个胚胎移植,51头受体接受双胚胎移植。受体在第16天之前可成功怀孕,但第17天则不行,表示胚胎必须在此阶段之前存在以防止黄体溶解。第42天的总体妊娠率为50.4%,另有18.1%的受体发情周期延长。在35头允许足月分娩的受体中,有12头流产,大多发生在第42天至63天之间。