Dennert G, Hyman R
Eur J Immunol. 1977 May;7(5):251-7. doi: 10.1002/eji.1830070502.
The induction and effector steps of T cell-mediated cytotoxicity (CMC) have been studied using a mouse tumor cell line and its variant, which is deficient in serologically defined (SD) H-2 antigens. In allogeneic mice the SDxcell line induces CMC, while the SD- cell line does not. However, both cell lines can be lysed by xenogeneic rat lymphocytes. Antiserum specific for rat T cells was used to demonstrate that CMC of both targetss is partially due to T cells. In allogeneic or syngeneic mouse systems the SD- cells coupled with the 2,4,6-trinitrophenyl (TNP) residue can neither induce CMC nor serve as targets for CMC, while TNP-coupled SDxcells can serve both as immunogen and as targets. Thus allogeneic or syngeneic mouse T cells do not interact with the TNP group of targets lacking H-2 SD antigens. However, mouse T killer cells sensitized to TNP-coupled cells may lyse TNP-coupled targets carrying different H-2 haplotypes. These experiments show that the induction and effector steps of CMC executed by mouse T cells, using TNP-coupled cells as immunogen or targets, need not necessarily demonstrate restriction with regard to a certain genetically defined H-2 haplotype. The presence of cell surface H-2 SD antigens is however, absolutely necessary for the induction and effector steps of CMC by mouse T cells. Using cold target inhibition assays, it was not possible to demonstrate recognition of the TNP moiety on TNP-coupled SDxcells.
利用一种小鼠肿瘤细胞系及其变体（该变体缺乏血清学定义的（SD）H-2抗原），对T细胞介导的细胞毒性（CMC）的诱导和效应步骤进行了研究。在同种异体小鼠中，SDx细胞系可诱导CMC，而SD-细胞系则不能。然而，两种细胞系均可被异种大鼠淋巴细胞裂解。使用针对大鼠T细胞的特异性抗血清来证明两种靶标的CMC部分归因于T细胞。在同种异体或同基因小鼠系统中，与2,4,6-三硝基苯基（TNP）残基偶联的SD-细胞既不能诱导CMC，也不能作为CMC的靶标，而TNP偶联的SDx细胞既可以作为免疫原，也可以作为靶标。因此，同种异体或同基因小鼠T细胞不与缺乏H-2 SD抗原的靶标的TNP基团相互作用。然而，对TNP偶联细胞致敏的小鼠T杀伤细胞可能会裂解携带不同H-2单倍型的TNP偶联靶标。这些实验表明，以TNP偶联细胞作为免疫原或靶标时，小鼠T细胞执行的CMC的诱导和效应步骤不一定表现出对某种基因定义的H-2单倍型的限制。然而，细胞表面H-2 SD抗原的存在对于小鼠T细胞进行CMC的诱导和效应步骤绝对必要。使用冷靶抑制试验，无法证明对TNP偶联的SDx细胞上的TNP部分有识别作用。
