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[玉米赤霉烯酮的生物转化]

[Biotransformation of zearalenone].

作者信息

Wu L, Wang Q, Wang S, Zhou F

机构信息

Shanghai Institute of Organic Chemistry, Academia Sinica.

出版信息

Wei Sheng Wu Xue Bao. 1992 Feb;32(1):11-6.

PMID:1413733
Abstract

The conversion of Zearalenone by some strains of microorganisms was investigated. When the selective strains of Rhodotorula sp., Arthrobacter sp., Saccharomyces sp., and Candida sp. were incubated by shaking or standing at 28 degrees C for 72 h with an alcoholic solution of Zearalenone as the substrate at a concentration of 2-10 mg/ml ethanol (50-100 micrograms/ml medium), it was readily converted to give Zearalenols, consisting either mainly of the alpha-isomer (e.g. 96% in case of Rhodotorula sp. and 84% in case of Arthrobacter sp. as determined by HPLC) or beta-isomer (e.g. 91% and 92% in Saccharomyces sp. and Candida sp., respectively). The structure of the product was confirmed by 13C-NMR, MS and HPLC.

摘要

研究了一些微生物菌株对玉米赤霉烯酮的转化情况。当将红酵母属、节杆菌属、酿酒酵母属和假丝酵母属的选择性菌株与浓度为2 - 10 mg/ml乙醇(50 - 100μg/ml培养基)的玉米赤霉烯酮醇溶液在28℃下振荡或静置培养72小时时,它很容易被转化为玉米赤霉烯醇,主要由α-异构体组成(例如,通过高效液相色谱法测定,红酵母属的情况为96%,节杆菌属的情况为84%)或β-异构体(例如,酿酒酵母属和假丝酵母属分别为91%和92%)。产物的结构通过13C - NMR、质谱和高效液相色谱法得到确认。

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