BORNSTEIN P, MARTIN G R, PIEZ K A
Science. 1964 Jun 5;144(3623):1220-1. doi: 10.1126/science.144.3623.1220.
Extraction of skin with 5M guanidine after salt and acid extraction yields a gelatin fraction which contains a greater proportion of double-chain (beta) components than can be accounted for by intramolecular cross-linking of collagen molecules. This fraction also contains a new beta-component, identified as the dimer of alpha2 and designated beta(22). This dimer must be formed by intermolecular crosslinking since each collagen molecule contains only one alpha2 chain. Thus, direct evidence is presented for the occurrence of both inter- and intramolecular crosslinking by what appears to be a single continuous process.
在用盐和酸提取后，用5M胍提取皮肤可得到一种明胶级分，该级分所含双链（β）组分的比例高于胶原蛋白分子内分子间交联所能解释的比例。该级分还含有一种新的β组分，鉴定为α2的二聚体，命名为β(22)。由于每个胶原蛋白分子仅含有一条α2链，所以这种二聚体必定是通过分子间交联形成的。因此，本文提供了直接证据，证明分子间和分子内交联似乎是通过一个单一的连续过程发生的。
