Friederichs E, Farley R A, Meiselman H J
Department of Physiology and Biophysics, University of Southern California, School of Medicine, Los Angeles 90033.
Am J Hematol. 1992 Nov;41(3):170-7. doi: 10.1002/ajh.2830410306.
The present study was designed to evaluate the influence of intracellular calcium [Ca]i regulated membrane attached hemoglobin (Hbm) on the deformability of human RBC and ghosts. [Ca]i of RBC was elevated via the ionophore A23187 (10 microM); the deformability of RBC and resealed ghosts was determined via measuring RBC and ghost transit times through 5 microns diameter pores with the Cell Transit Analyzer (CTA). Salient results included: (1) significantly increased RBC levels of Hbm following ionophore treatment; (2) elevated Hbm with increasing lysing medium calcium concentration (0-5 mM); (3) decreased deformability of both intact RBC and ghosts with increasing Hbm and significant (P less than 0.02 or better) linear relationships between Hbm and RBC or ghost transit times; and (4) an increased sensitivity to ionophore treatment/membrane attached hemoglobin for the higher percentiles of the CTA transit time distribution (i.e., for more rigid subpopulations). Our results thus indicate that calcium-induced interaction of hemoglobin with the RBC membrane produces cellular rheological changes; in addition, they demonstrate the usefulness of the CTA system in measuring both average RBC rheologic behavior and the distribution of cellular rheologic properties within an erythrocyte population.
本研究旨在评估细胞内钙[Ca]i调节的膜附着血红蛋白（Hbm）对人红细胞和血影变形性的影响。通过离子载体A23187（10微摩尔）升高红细胞的[Ca]i；通过使用细胞通过分析仪（CTA）测量红细胞和血影通过5微米直径小孔的通过时间来确定红细胞和重封血影的变形性。主要结果包括：（1）离子载体处理后红细胞Hbm水平显著升高；（2）随着裂解介质钙浓度（0 - 5毫摩尔）增加，Hbm升高；（3）随着Hbm增加，完整红细胞和血影的变形性降低，且Hbm与红细胞或血影通过时间之间存在显著（P小于0.02或更佳）的线性关系；（4）对于CTA通过时间分布的较高百分位数（即对于更刚性的亚群），对离子载体处理/膜附着血红蛋白的敏感性增加。因此，我们的结果表明钙诱导的血红蛋白与红细胞膜的相互作用产生细胞流变学变化；此外，它们证明了CTA系统在测量红细胞平均流变行为和红细胞群体内细胞流变特性分布方面的有用性。
