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人类红细胞中蛋白质特性的温度转变

Temperature transitions of protein properties in human red blood cells.

作者信息

Artmann G M, Kelemen C, Porst D, Büldt G, Chien S

机构信息

Department of Cell Biophysics, Aachen University of Applied Sciences, Ginsterweg 1, D-52428 Jülich, Germany.

出版信息

Biophys J. 1998 Dec;75(6):3179-83. doi: 10.1016/S0006-3495(98)77759-8.

DOI:10.1016/S0006-3495(98)77759-8
PMID:9826638
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1299989/
Abstract

Human red blood cells (RBC) undergo a sudden change from blocking to passing through 1.3 +/- 0.2-micrometer micropipettes at a transition temperature (Tc) of 36.4 degrees C. For resealed RBC ghosts this transition occurs at 28.3 degrees C (Tg). These findings are attributed to an elastomeric transition of hemoglobin from being gel-like to a fluid and to an elastomeric transition of membrane proteins such as spectrin. Spectrin shows a uniform distribution along the aspirated RBC tongue above Tg in contrast to the linear gradient below Tg.

摘要

人类红细胞(RBC)在36.4摄氏度的转变温度(Tc)下,会突然从阻塞状态转变为能够通过1.3±0.2微米的微量移液器。对于重新密封的红细胞空壳,这种转变发生在28.3摄氏度(Tg)。这些发现归因于血红蛋白从凝胶状到流体状的弹性转变,以及诸如血影蛋白等膜蛋白的弹性转变。与Tg以下的线性梯度相反,血影蛋白在Tg以上沿吸出的红细胞舌呈现均匀分布。

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