Tee M K, Moran C, Nicholas F W
Department of Animal Science, University of Sydney, New South Wales, Australia.
Anim Genet. 1992;23(5):431-5. doi: 10.1111/j.1365-2052.1992.tb02162.x.
An A in equilibrium with G transition in exon III is known to differentiate alleles A and B of the cattle beta-lactoglobulin (BLG) gene. A BLG exon III fragment containing the transition site was amplified by the polymerase chain reaction. Temperature gradient gel electrophoresis (TGGE) was then used to detect this transition and hence to genotype cattle: the AT base-pair in allele A was readily distinguished from the GC base-pair of allele B. TGGE can be used to detect any single base-pair substitution, and thus is a powerful method of detecting genetic variability.
已知外显子III中A与G的平衡转换可区分牛β-乳球蛋白（BLG）基因的等位基因A和B。通过聚合酶链反应扩增包含该转换位点的BLG外显子III片段。然后使用温度梯度凝胶电泳（TGGE）检测这种转换，从而对牛进行基因分型：等位基因A中的AT碱基对很容易与等位基因B中的GC碱基对区分开来。TGGE可用于检测任何单碱基对替换，因此是检测遗传变异性的一种强大方法。
