STAPHYLOCOCCAL ALPHA-HEMOLYSIN: DETECTION ON THE ERYTHROCYTE MEMBRANE BY IMMUNOFLUORESCENCE.

Purified staphylococcal alpha-hemolysin (but not the toxoid) was demonstrated on the surface of rabbit and human erythrocytes by immunofluorescence. This occurred during the period of maximal hemolysis and was a transient event. These findings have been analyzed in relation to previous data on the kinetics of leakage of both small and complex molecular constituents of the erythrocyte.