Klainer A S, Chang T W, Weinstein L
Infect Immun. 1972 May;5(5):808-13. doi: 10.1128/iai.5.5.808-813.1972.
Previous studies have suggested that the primary site of action of purified staphylococcal alpha toxin is the cell membrane. Scanning and transmission electron microscopy studies were undertaken, therefore, to define toxin-induced alterations in the surface morphology of rabbit and human red blood cells. During the prelytic lag phase, scanning electron microscopy revealed multiple discrete blisters on the surface of rabbit red blood cells; during hemolysis, cellular collapse and ghosts were seen, but most striking was the separation of large fragments of cell membrane from red blood cell surfaces. In contrast, alterations in less sensitive human red blood cells were limited to occasional fingerlike protrusions during the period of accelerated lysis. Transmission electron microscopy substantiated these changes. These studies have provided further evidence that the cell membrane is the primary site of action of staphylococcal alpha toxin.
以往的研究表明，纯化的葡萄球菌α毒素的主要作用部位是细胞膜。因此，进行了扫描电子显微镜和透射电子显微镜研究，以确定毒素诱导的兔和人红细胞表面形态的变化。在溶血前的延迟期，扫描电子显微镜显示兔红细胞表面有多个离散的水泡；在溶血过程中，可见细胞塌陷和血影，但最显著的是细胞膜的大片段从红细胞表面分离。相比之下，不太敏感的人红细胞的变化仅限于加速溶血期间偶尔出现的指状突起。透射电子显微镜证实了这些变化。这些研究进一步证明细胞膜是葡萄球菌α毒素的主要作用部位。
