Metzler G, Schaumburg-Lever G, Fehrenbacher B, Möller H
Department of Dermatology, Eberhard-Karls-Universität, Tübingen, Federal Republic of Germany.
Arch Dermatol Res. 1992;284(4):242-5. doi: 10.1007/BF00375802.
Normal human skin was embedded in Lowicryl K4M. Actin microfilaments were localized by applying a postembedding immunogold technique using the monoclonal anti-actin antibody HHF35. Actin microfilaments are part of the cytoskeleton in muscle and nonmuscle cells. Together with myosin they produce contraction. The antibody labelled myofilaments in smooth muscle arrector pili cells, myoepithelial cells and pericytes. In sweat gland cells the microvilli system, a zone beneath the cytoplasma membrane corresponding to the adhesion belt region, and apocrine decapitation formations showed labelling.
将正常人皮肤包埋于Lowicryl K4M中。采用后包埋免疫金技术，使用抗肌动蛋白单克隆抗体HHF35定位肌动蛋白微丝。肌动蛋白微丝是肌肉细胞和非肌肉细胞细胞骨架的一部分。它们与肌球蛋白一起产生收缩作用。该抗体标记了平滑肌立毛肌细胞、肌上皮细胞和周细胞中的肌丝。在汗腺细胞中，微绒毛系统、细胞膜下方对应于黏着带区域的一个区域以及顶浆分泌断头结构均显示有标记。
