de Kater A W, Shahsafaei A, Epstein D L
Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston.
Invest Ophthalmol Vis Sci. 1992 Feb;33(2):424-9.
alpha-Smooth muscle actin is the isoform of actin restricted to vascular smooth muscle, pericytes, myofibroblasts and, certain other cells that are of myoid origin. We investigated the distribution of alpha-smooth muscle actin and nonmuscle specific filamentous actin in the human aqueous outflow system by immunohistochemical methods. Filamentous actin was observed in all cellular constituents of the outflow pathway, while distribution of alpha-smooth muscle actin was restricted to the ciliary muscle, to specific cells throughout the trabecular meshwork, and to cells adjacent to the outer wall and the collector channels. The ciliary muscle extended deep into the corneoscleral meshwork, far anterior to the scleral spur. These findings agree with our previous study localizing the distribution of smooth muscle myosin in the human aqueous outflow pathway. Although functionality of the immunoreactive cells needs to be demonstrated, our data show that a potentially contractile apparatus exists in a subpopulation of trabecular meshwork cells and in certain cells of the more distal components of the outflow system.
α-平滑肌肌动蛋白是肌动蛋白的一种同工型，仅限于血管平滑肌、周细胞、肌成纤维细胞以及某些其他起源于肌样的细胞。我们通过免疫组织化学方法研究了α-平滑肌肌动蛋白和非肌肉特异性丝状肌动蛋白在人房水流出系统中的分布。在流出途径的所有细胞成分中均观察到丝状肌动蛋白，而α-平滑肌肌动蛋白的分布仅限于睫状肌、小梁网中的特定细胞以及与外壁和集合管相邻的细胞。睫状肌深入到角膜巩膜小梁网，远在巩膜突前方。这些发现与我们之前关于平滑肌肌球蛋白在人房水流出途径中分布的研究结果一致。尽管免疫反应性细胞的功能需要得到证实，但我们的数据表明，在小梁网细胞亚群和流出系统更远端成分的某些细胞中存在潜在的收缩装置。
