Fattaey A, Lenz L, Consigli R A
Division of Biology, Kansas State University, Manhattan 66506.
Avian Dis. 1992 Jul-Sep;36(3):543-53.
Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies was reactive in the immunofluorescent detection of BFD virus-infected cells. These antibodies immunoprecipitated intact virions and specifically recognized the major capsid protein, VP1, of the dissociated virion. The MAbs were found to preferentially recognize native BFD virus capsid protein when compared with denatured virus protein. These MAbs were capable of detecting BFD virus protein in chicken embryonated cell-culture lysates by dot-blot analysis.
产生针对完整虎皮鹦鹉幼雏病（BFD）病毒粒子的单克隆抗体（MAb）的11个杂交瘤细胞系被制备并进行了特性鉴定。这些抗体因在酶联免疫吸附测定中与BFD病毒粒子发生反应的能力而被挑选出来。这些抗体中的每一种在BFD病毒感染细胞的免疫荧光检测中都具有反应性。这些抗体免疫沉淀完整的病毒粒子，并特异性识别解离病毒粒子的主要衣壳蛋白VP1。与变性病毒蛋白相比，发现这些单克隆抗体优先识别天然BFD病毒衣壳蛋白。通过斑点印迹分析，这些单克隆抗体能够检测鸡胚细胞培养裂解物中的BFD病毒蛋白。
