Smiley S A, Gillock E T, Black M C, Consigli R A
Division of Biology--Ackert Hall, Section of Virology and Oncology, Kansas State University, Manhattan 66506, USA.
Exp Cell Res. 1997 Feb 1;230(2):411-4. doi: 10.1006/excr.1996.3445.
The hybridoma cell line, 3G10G5, producing a monoclonal antibody to the major capsid protein VP1 from the avian polyomavirus budgerigar fledgling disease virus, was produced from a Balb/C mouse. This cell line was used to test the effects of microgravity on cellular processes, specifically protein synthesis. A time course study utilizing incorporation of [35S]methionine into newly synthesized monoclonal antibody was performed on STS-77. After 5.5 days, it was observed that cell counts for the samples exposed to microgravity were lower than those of ground-based samples. However, radiolabel incorporation of the synthesized monoclonal antibody was similar in both orbiter and ground control samples. Overall, microgravity does not seem to have an effect on this cell line's ability to synthesize IgG protein.
杂交瘤细胞系3G10G5由Balb/C小鼠制备而成，该细胞系可产生针对禽多瘤病毒虎皮鹦鹉幼雏病病毒主要衣壳蛋白VP1的单克隆抗体。此细胞系用于测试微重力对细胞过程，特别是蛋白质合成的影响。利用[35S]甲硫氨酸掺入新合成的单克隆抗体进行了一项时间进程研究，该研究在STS - 77上进行。5.5天后，观察到暴露于微重力环境下的样品细胞计数低于地面样品。然而，合成的单克隆抗体的放射性标记掺入在轨道器样品和地面对照样品中相似。总体而言，微重力似乎对该细胞系合成IgG蛋白的能力没有影响。
