一种与牛前速激肽原启动子内序列相互作用的单链DNA结合蛋白：受神经生长因子调控。

A single-stranded DNA binding protein which interacts with sequences within the bovine preprotachykinin promoter: regulation by nerve growth factor.

作者信息

Gilchrist C A, Morrison C F, Harmar A J

机构信息

MRC Brain Metabolism Unit, Royal Edinburgh Hospital, Scotland.

出版信息

Biochem Biophys Res Commun. 1992 Sep 30;187(3):1395-400. doi: 10.1016/0006-291x(92)90457-v.

DOI:10.1016/0006-291x(92)90457-v

PMID:1417815

Abstract

The production of substance P (SP) and the mRNA encoding its precursor preprotachykinin (PPT) is regulated by nerve growth factor (NGF) in dorsal root ganglion neurons. We have shown previously that two regions of the bovine PPT promoter are capable of mediating the induction by NGF of the downstream structural gene in transfected PC12 cells. Both regions contained a sequence element, similar to a known transcription factor binding site, which is present in several other NGF-regulated genes. We show here that PC12 cells contain a single-stranded DNA binding protein (SSBP-PC12) which can interact specifically with this site. Binding activity was increased by NGF treatment of PC12 cells.

摘要

在背根神经节神经元中，P物质（SP）及其编码前体前速激肽原（PPT）的mRNA的产生受神经生长因子（NGF）调控。我们先前已表明，牛PPT启动子的两个区域能够介导转染的PC12细胞中NGF对下游结构基因的诱导作用。这两个区域都含有一个序列元件，类似于一个已知的转录因子结合位点，该位点也存在于其他几个受NGF调控的基因中。我们在此表明，PC12细胞含有一种单链DNA结合蛋白（SSBP - PC12），它能与该位点特异性相互作用。用NGF处理PC12细胞可增加结合活性。