Rubini M, Selvatici R, Orlando P, Balboni A, Boninsegna S, Gandini E
Institute of Medical Genetics, University of Ferrara, Italy.
Biochem Biophys Res Commun. 1992 Oct 15;188(1):278-85. doi: 10.1016/0006-291x(92)92381-7.
It is known that anti-HLA Class I antibodies inhibit the proliferative response of PHA-activated T-lymphocytes. We found that plastic- or sepharose-linked anti-HLA Class I monoclonal antibody 01.65 does not inhibit either [3H]Thymidine incorporation or recruitment in the cell cycle, nor does it reduce the expression of c-myc mRNA and the membrane expression of Interleukin-2 Receptor and Transferrin Receptor. Furthermore, particulate Protein Kinase C is not affected by anchored anti-HLA Class I monoclonal antibody 01.65. We suggest that anti-HLA Class I monoclonal antibody may act through crosslinking or internalization of HLA Class I antigens.