锚定的抗HLA I类单克隆抗体未能诱导对PHA激活的淋巴细胞增殖的抑制作用。
Anchored anti-HLA class I monoclonal antibody fails to induce inhibition of PHA-activated lymphocytes proliferation.
作者信息
Rubini M, Selvatici R, Orlando P, Balboni A, Boninsegna S, Gandini E
机构信息
Institute of Medical Genetics, University of Ferrara, Italy.
出版信息
Biochem Biophys Res Commun. 1992 Oct 15;188(1):278-85. doi: 10.1016/0006-291x(92)92381-7.
It is known that anti-HLA Class I antibodies inhibit the proliferative response of PHA-activated T-lymphocytes. We found that plastic- or sepharose-linked anti-HLA Class I monoclonal antibody 01.65 does not inhibit either [3H]Thymidine incorporation or recruitment in the cell cycle, nor does it reduce the expression of c-myc mRNA and the membrane expression of Interleukin-2 Receptor and Transferrin Receptor. Furthermore, particulate Protein Kinase C is not affected by anchored anti-HLA Class I monoclonal antibody 01.65. We suggest that anti-HLA Class I monoclonal antibody may act through crosslinking or internalization of HLA Class I antigens.
已知抗HLA I类抗体可抑制PHA激活的T淋巴细胞的增殖反应。我们发现，与塑料或琼脂糖结合的抗HLA I类单克隆抗体01.65既不抑制[3H]胸苷掺入或细胞周期中的募集，也不降低c-myc mRNA的表达以及白细胞介素-2受体和转铁蛋白受体的膜表达。此外，颗粒状蛋白激酶C不受固定的抗HLA I类单克隆抗体01.65的影响。我们认为抗HLA I类单克隆抗体可能通过HLA I类抗原的交联或内化发挥作用。
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