抗HLA I类单克隆抗体对PHA激活的人外周血单个核细胞和E+细胞中蛋白激酶C动力学的影响。
Anti HLA class I monoclonal antibody effect on PKC kinetics in PHA activated human peripheral blood mononuclear and E+ cells.
作者信息
Poltronieri L, Melloni E, Rubini M, Selvatici R, Mazzilli C, Baricordi R, Gandini E
机构信息
Istituto di Genetica Medica, Universita' degli Studi di Ferrara, Italy.
出版信息
Biochem Biophys Res Commun. 1988 Oct 14;156(1):46-53. doi: 10.1016/s0006-291x(88)80803-9.
Cytoplasmic protein kinase C (PKC) has been studied in phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMC) and macrophage depleted E+ cell culture. Within 10' after contemporanous addition of PHA and anti HLA class I monoclonal antibody 01.65 (MoAb) PKC is depleted in both cell types. Enzyme activity recovers in the following hours however at 72 hours is at control values in E+ cultures while in PBMC cultures it is still depleted at 68% of the control. Anti HLA class I MoAb induced tritiated lymidine (3H-TdR) incorporation inhibition appears to be related to low levels of PKC activity.
已在植物血凝素（PHA）激活的外周血单个核细胞（PBMC）和巨噬细胞耗竭的E +细胞培养物中研究了细胞质蛋白激酶C（PKC）。在同时添加PHA和抗HLA I类单克隆抗体01.65（MoAb）后10分钟内，两种细胞类型中的PKC均被耗尽。然而，酶活性在接下来的几个小时内恢复，在E +培养物中72小时时达到对照值，而在PBMC培养物中，在72小时时仍以对照值的68%被耗尽。抗HLA I类MoAb诱导的氚化胸腺嘧啶核苷（3H-TdR）掺入抑制似乎与低水平的PKC活性有关。
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