Turinsky J, Nagel G W
Department of Physiology and Cell Biology, Albany Medical College, NY 12208.
Biochem Biophys Res Commun. 1992 Oct 15;188(1):358-64. doi: 10.1016/0006-291x(92)92393-c.
Incubation of rat soleus muscles with 50 microM sphingosine or 50 microM sphinganine augmented basal 2-deoxy-D-glucose (2DG) transport 32%, but reduced the response to 0.1 and 1.0 mU insulin/ml by 17 and 27%, respectively. When the muscles were incubated with 50 microM phytosphingosine, a 63-93% increase in basal 2DG transport was observed. However, this treatment had no effect on insulin-stimulated 2DG transport. The phytosphingosine-induced increase in basal 2-DG transport was inhibited 93 and 98% with 35 and 70 microM cytochalasin B, respectively, suggesting that it is mediated by glucose transporters. Cellular accumulation of L-glucose, which is not mediated by glucose transporters, was not affected by phytosphingosine. It is concluded that (a) both sphingosine and sphinganine increase basal 2DG transport in muscle but diminish insulin-stimulated transport, and (b) phytosphingosine stimulates basal 2DG transport in muscle by a mechanism involving glucose transporters.
将大鼠比目鱼肌与50微摩尔的鞘氨醇或50微摩尔的二氢鞘氨醇一起孵育，可使基础2-脱氧-D-葡萄糖（2DG）转运增加32%，但对每毫升0.1和1.0毫单位胰岛素的反应分别降低了17%和27%。当肌肉与50微摩尔的植物鞘氨醇一起孵育时，观察到基础2DG转运增加了63 - 93%。然而，这种处理对胰岛素刺激的2DG转运没有影响。分别用35和70微摩尔的细胞松弛素B处理时，植物鞘氨醇诱导的基础2-DG转运增加分别被抑制了93%和98%，这表明它是由葡萄糖转运体介导的。L-葡萄糖的细胞积累不受植物鞘氨醇的影响，因为L-葡萄糖的积累不是由葡萄糖转运体介导的。结论是：（a）鞘氨醇和二氢鞘氨醇都增加肌肉中的基础2DG转运，但会减少胰岛素刺激的转运；（b）植物鞘氨醇通过一种涉及葡萄糖转运体的机制刺激肌肉中的基础2DG转运。
