Usta J, Griffiths D E
Department of Chemistry, University of Warwick, Coventry, UK.
Biochem Biophys Res Commun. 1992 Oct 15;188(1):365-71. doi: 10.1016/0006-291x(92)92394-d.
Fluorescent 5-coordinate organotin-flavone complexes of 3-hydroxy-flavone (Hof) and 3,5,7,2',4',-pentahydroxyflavone (morin) are good inhibitors of mitochondrial F1F0ATPase but do not inhibit F1-ATPase and they have been examined as possible fluorescent probes of F1F0ATPase. R2SnX (morin) complexes exhibit low fluorescence enhancement on binding to mitochondrial membranes with no displacement by equimolar tributyltin. In contrast R2SnX (of) complexes exhibit high fluorescence enhancement whose extent is variable and is displacable by equimolar tributyltin. Fluorescence enhancement by R2SnX (of) complexes correlates with the ATPase I50 values. Dialkyltin-3-hydroxy flavone, R2SnX(of), complexes act as a new class of fluorescent probes which titrate the F0 segment of F1F0ATPase.
3-羟基黄酮（Hof）和3,5,7,2',4'-五羟基黄酮（桑色素）的荧光五配位有机锡-黄酮配合物是线粒体F1F0ATP酶的良好抑制剂，但不抑制F1-ATP酶，并且它们已被作为F1F0ATP酶可能的荧光探针进行了研究。R2SnX（桑色素）配合物在与线粒体膜结合时荧光增强较低，等摩尔的三丁基锡不能将其取代。相比之下，R2SnX（Hof）配合物表现出较高的荧光增强，其程度可变且可被等摩尔的三丁基锡取代。R2SnX（Hof）配合物的荧光增强与ATP酶的I50值相关。二烷基锡-3-羟基黄酮，R2SnX（Hof）配合物作为一类新型荧光探针，可滴定F1F0ATP酶的F0片段。
