Prasad P D, Malhotra P, Karande A A, Adiga P R
Centre for Reproductive Biology & Molecular Endocrinology, Indian Institute of Science, Bangalore.
Biochem Int. 1992 Jul;27(3):385-95.
A specific protein exhibiting immunological cross-reactivity with chicken riboflavin carrier protein has been purified to homogeneity from human amniotic fluid by use of ion-exchange and affinity chromatography. The protein is similar to its avian counterpart in terms of molecular size, distribution of 125I-labelled tryptic peptides during finger printing, and preferential binding to riboflavin. Immunologically, they are homologous since most of the monoclonal antibodies raised against the avian protein cross-react with the purified human vitamin carrier.
通过离子交换和亲和层析法，从人羊水中纯化出一种与鸡核黄素载体蛋白具有免疫交叉反应性的特定蛋白质，该蛋白质已达到均一性。该蛋白质在分子大小、指纹图谱中¹²⁵I标记胰蛋白酶肽的分布以及与核黄素的优先结合方面与其禽类对应物相似。在免疫学上，它们是同源的，因为针对禽类蛋白质产生的大多数单克隆抗体与纯化的人维生素载体发生交叉反应。
