Hashim O H, Kobayashi K, Taniguchi N
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur.
Biochem Int. 1992 Jul;27(3):423-9.
In view of the controversy with respect to the interaction of jacalin with human IgA2, a study was undertaken to assess the reactivity of the Artocarpus heterophyllus lectin, as well as the lectin from Artocarpus integer (lectin C), with subclasses of human immunoglobulin A by ELISA. Our data is consistent with the view that Artocarpus lectins have no affinity for the IgA2 immunoglobulins. In further support of the findings, we have established that N-linked oligosaccharide moieties of IgA have no significant bearing in the lectin-immunoglobulin binding. Interaction was also not affected in the presence of 1% (w/v) BSA.
鉴于关于jacalin与人类IgA2相互作用存在争议，开展了一项研究，通过酶联免疫吸附测定法评估面包果凝集素以及尖叶桂木凝集素（凝集素C）与人类免疫球蛋白A亚类的反应性。我们的数据与面包果凝集素对IgA2免疫球蛋白无亲和力这一观点一致。为进一步支持这些发现，我们已确定IgA的N-连接寡糖部分在凝集素-免疫球蛋白结合中无显著影响。在存在1%（w/v）牛血清白蛋白的情况下，相互作用也未受影响。
