Perocco P, Ferreri A M, Franceschi C, Grilli S, Rocchi P, Arfellini G, Prodi G
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1977 May 20;89(1):99-106. doi: 10.1007/BF02571694.
The effect of N-hydroxyurethan (HUR) on DNA synthesis has been tested both in vivo on various tissues and in vitro on concanavalin A (ConA)-stimulated rat thymocytes and compared with the action of urethan and hydroxyurea. HUR suppresses scheduled DNA synthesis, except that of non-stimulated spleen cells in vitro. The inhibition is efficient and rapid and takes place immediately if the drug is administered at the peak of the S-phase. UR inhibits DNA synthesis in vitro only at much higher doses and with different time course. It is effective or slightly effective if it is administered at the peak of the S-phase. A conversion of urethan into HUR the latter depressing DNA synthesis could partly explain the differences observed. No toxicity was found after treatment with drugs at the concentration employed. Finally, the relationships between drug doses and cell responses have been particularly observed in vivo.
已在体内对多种组织以及体外对伴刀豆球蛋白A（ConA）刺激的大鼠胸腺细胞测试了N-羟基脲（HUR）对DNA合成的影响，并与脲烷和羟基脲的作用进行了比较。HUR抑制预定的DNA合成，但体外未刺激的脾细胞除外。这种抑制作用高效且迅速，如果在S期峰值时给药，抑制作用会立即发生。脲烷仅在高得多的剂量下且具有不同的时间进程时才会在体外抑制DNA合成。如果在S期峰值时给药，它有效或效果轻微。脲烷转化为HUR，后者抑制DNA合成，这可能部分解释了观察到的差异。在所使用的浓度下用药物处理后未发现毒性。最后，在体内特别观察了药物剂量与细胞反应之间的关系。
