Franceschi C, Perocco P, Paolucci P, Prodi G
Int Arch Allergy Appl Immunol. 1976;50(5):513-24. doi: 10.1159/000231556.
In vitro phytohemagglutinin (PHA) and concanavalin A (Con A) responsiveness of thymus and bone marrow cells, and E, EA, and EAC rosette-forming cells (E, EA, EAC) of these organs and spleen were studied in Fisher rats at various time intervals following a carcinogenic treatment with urethan (UR). Immediately after treatment, organ cellularities were drastically reduced with a progressive recovery as time passed. 1 day after treatment, the response of thymocytes to PHA showed a fourfold increase while organ cellularity dropped to 1%. This response fell below normal values after 7 days with an overshoot after days 14 and 21. UR-treated bone marrow cells showed a response to PHA below normal values after 1 and 7 days, a twofold increase after 3 days and an overshoot after 14 and 21 days. The responsiveness to Con A of both organs was affected by UR treatment to a much lesser extent, although following a pattern similar to that of PHA. E were never found, whether in normal nor in UR-treated animals. As far as the B-cell compartment is concerned, UR causes a progressive diminution of EAC, while the number of EA remains unaffected. The data are discussed in terms of selective effect on T- and B-cell subpopulations.
在用氨基甲酸乙酯（UR）进行致癌处理后的不同时间间隔，研究了Fisher大鼠胸腺和骨髓细胞对体外植物血凝素（PHA）和刀豆球蛋白A（Con A）的反应性，以及这些器官和脾脏的E、EA和EAC玫瑰花结形成细胞（E、EA、EAC）。处理后立即观察到，器官细胞数量急剧减少，随后随着时间推移逐渐恢复。处理后1天，胸腺细胞对PHA的反应增加了四倍，而器官细胞数量降至1%。7天后该反应低于正常值，在14天和21天后出现过冲现象。UR处理的骨髓细胞在1天和7天后对PHA的反应低于正常值，3天后增加两倍，14天和21天后出现过冲现象。尽管两种器官对Con A的反应模式与对PHA的相似，但UR处理对其影响程度要小得多。无论是在正常动物还是UR处理的动物中，均未发现E。就B细胞区室而言，UR导致EAC逐渐减少，而EA数量不受影响。从对T细胞和B细胞亚群的选择性作用方面对这些数据进行了讨论。
