Hochi S, Ninomiya T, Waga-Homma M, Sagara J, Yuki A
Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., Tochigi, Japan.
Mol Reprod Dev. 1992 Oct;33(2):160-4. doi: 10.1002/mrd.1080330207.
Bovine alpha-lactalbumin (b alpha LA) gene prepared by polymerase chain reaction was introduced into rat genomes by microinjection. Out of 17 transgenic rat lines, 11 secreted b alpha LA into their milk at concentrations higher than 0.2 micrograms/ml. Of these, three lines secreted b alpha LA at concentrations higher than those in bovine milk. The highest concentration of b alpha LA secreted into rat milk was 2,400 micrograms/ml.
通过聚合酶链反应制备的牛α-乳白蛋白（bαLA）基因经显微注射导入大鼠基因组。在17个转基因大鼠品系中，有11个品系能以高于0.2微克/毫升的浓度在其乳汁中分泌bαLA。其中，有3个品系分泌bαLA的浓度高于牛乳中的浓度。大鼠乳汁中分泌的bαLA的最高浓度为2400微克/毫升。
