STRAUS W
J Cell Biol. 1964 Jun;21(3):295-308. doi: 10.1083/jcb.21.3.295.
The size, number, and location of lysosomes, phagosomes, and phago-lysosomes in different segments of the proximal and distal tubules, in the collecting tubules, and in invading macrophages of the kidneys of rats were compared by staining lysosomes (acid phosphatase) red, and phagosomes (injected horseradish peroxidase) blue in separate sections, and by staining phago-lysosomes purple by successive application of the reactions for the two enzymes in the same sections. It was concluded from these observations that the absorption of the foreign protein from the lumen and its gradual digestion in large phago-lysosomes took place mainly in the cells of the proximal convoluted tubules of the outer cortex. Several segments of the proximal convoluted tubules were distinguished on the basis of differences in the size and location of the phago-lysosomes and the amounts of peroxidase ingested. The distal tubules showed, in addition to moderate numbers of phago-lysosomes, many small phagosomes in the apical and basal zones of the cells. Moderate numbers of phagosomes and phago-lysosomes were observed in the cells of the collecting tubules. Macrophages showing very large phago-lysosomes were seen in the peritubular capillaries of the medulla, after injection of peroxidase. When high doses of peroxidase were administered, enlarged phago-lysosomes, parts of which seemed to be extruded into the lumen, were formed in the terminal segments of the proximal convoluted tubules.
通过在不同切片中分别将溶酶体（酸性磷酸酶）染成红色、吞噬体（注射辣根过氧化物酶）染成蓝色，并在同一切片中依次应用两种酶的反应将吞噬溶酶体染成紫色，比较了大鼠肾脏近端和远端小管不同节段、集合小管以及侵入的巨噬细胞中溶酶体、吞噬体和吞噬溶酶体的大小、数量和位置。从这些观察结果得出结论，外来蛋白质从管腔的吸收及其在大型吞噬溶酶体中的逐渐消化主要发生在外皮质近端曲管的细胞中。根据吞噬溶酶体的大小和位置以及摄取的过氧化物酶量的差异，区分了近端曲管的几个节段。除了数量适中的吞噬溶酶体外，远端小管在细胞的顶端和基部区域还显示出许多小吞噬体。在集合小管的细胞中观察到数量适中的吞噬体和吞噬溶酶体。注射过氧化物酶后，在髓质的肾小管周围毛细血管中可见显示非常大的吞噬溶酶体的巨噬细胞。当给予高剂量的过氧化物酶时，在近端曲管的末端节段形成了扩大的吞噬溶酶体，其中部分似乎被挤出到管腔中。
