豚草过敏患者皮肤致敏抗体的沉降特性
THE SEDIMENTATION PROPERTIES OF THE SKIN-SENSITIZING ANTIBODIES OF RAGWEED-SENSITIVE PATIENTS.
作者信息
ANDERSEN B R, VANNIER W E
出版信息
J Exp Med. 1964 Jul 1;120(1):31-43. doi: 10.1084/jem.120.1.31.
Abstract
The sedimentation coefficients of the skin-sensitizing antibodies to ragweed were evaluated by the moving partition cell method and the sucrose density gradient method. The most reliable results were obtained by sucrose density gradient ultracentrifugation which showed that the major portion of skin-sensitizing antibodies to ragweed sediment with an average value of 7.7S (7.4 to 7.9S). This is about one S unit faster than gamma-globulins (6.8S). The data from the moving partition cell method are in agreement with these results. Our studies failed to demonstrate heterogeneity of the skin-sensitizing antibodies with regard to sedimentation rate.
摘要
采用移动分配池法和蔗糖密度梯度法评估了豚草皮肤致敏抗体的沉降系数。通过蔗糖密度梯度超速离心获得了最可靠的结果，结果显示豚草皮肤致敏抗体的主要部分沉降，平均值为7.7S（7.4至7.9S）。这比γ-球蛋白（6.8S）快约一个S单位。移动分配池法的数据与这些结果一致。我们的研究未能证明皮肤致敏抗体在沉降速率方面存在异质性。
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