链球菌溶血素O：沉降系数及分子量的测定
Streptolysin O: sedimentation coefficient and molecular weight determinations.
作者信息
Van Epps D E, Andersen B R
出版信息
J Bacteriol. 1969 Oct;100(1):526-7. doi: 10.1128/jb.100.1.526-527.1969.
Abstract
The sedimentation coefficient of streptolysin O as determined by sucrose density gradient ultracentrifugation is 3.7S. An approximate molecular weight of 60,500 was calculated from the sedimentation velocity, and a similar value was obtained by Sephadex gel filtration. There was no measurable difference in the sedimentation coefficient of streptolysin O in either the active or reversibly inactive forms, indicating that there were at most only minor conformational differences between the two forms.
摘要
通过蔗糖密度梯度超速离心法测定，链球菌溶血素O的沉降系数为3.7S。根据沉降速度计算出其近似分子量为60,500，通过葡聚糖凝胶过滤法也得到了类似的值。无论是活性形式还是可逆失活形式的链球菌溶血素O，其沉降系数均无显著差异，这表明两种形式之间至多只有微小的构象差异。
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