FALANGHE H, SMITH A K, RACKIS J J
Appl Microbiol. 1964 Jul;12(4):330-4. doi: 10.1128/am.12.4.330-334.1964.
Various soybean whey media were tested as substrate for seven species of fungi in submerged culture. Very little mycelial growth was obtained with Morchella hybrida, Collybia velutipes, Cantharellus cibarius, and Xylaria polymorpha. Agaricus campestris failed to grow. Tricholoma nudum and Boletus indecisus showed the greatest rate of growth and production of mycelial protein and the best utilization of soybean whey solids, with much shorter incubation times compared with those of the other species. T. nudum developed as spheres having diameters of about 5 to 8 mm, instead of the usual slurry or yeastlike form, in the presence of added ammonium acetate. B. indecisus always developed as spheres. Mycelial yields and production of protein by T. nudum greatly decreased with the addition of more than 1% glucose to soybean whey, whereas with B. indecisus the yield of protein almost doubled when up to 3% glucose was added. The effect of minerals on mycelial growth was determined. With soybean whey concentrated to 50%, the rate of mycelial growth of T. nudum was nearly doubled, but protein content of mycelia was greatly reduced. Mycelial growth and yield of protein of B. indecisus grown in concentrated whey were increased greatly. About 4 to 6 g of mycelial protein per liter can be obtained from fermentation in soybean whey, depending upon the medium used. Utilization of soybean whey by fungal fermentation may have economic value in whey disposal and in the production of products of high protein content.
对多种大豆乳清培养基作为七种真菌深层培养底物进行了测试。用混合羊肚菌、毛脚鸡枞、鸡油菌和多形炭角菌几乎未获得菌丝体生长。双孢蘑菇未能生长。裸口蘑和可疑牛肝菌显示出最高的生长速率和菌丝体蛋白产量，并且对大豆乳清固体的利用率最佳，与其他物种相比培养时间短得多。在添加醋酸铵的情况下，裸口蘑形成直径约5至8毫米的球体，而不是通常的浆液或酵母样形态。可疑牛肝菌总是形成球体。向大豆乳清中添加超过1%的葡萄糖会使裸口蘑的菌丝体产量和蛋白产量大幅下降，而对于可疑牛肝菌，添加高达3%的葡萄糖时蛋白产量几乎翻倍。测定了矿物质对菌丝体生长的影响。当大豆乳清浓缩至50%时，裸口蘑的菌丝体生长速率几乎翻倍，但菌丝体的蛋白含量大幅降低。在浓缩乳清中生长的可疑牛肝菌的菌丝体生长和蛋白产量大幅增加。根据所用培养基的不同，每升大豆乳清发酵可获得约4至6克菌丝体蛋白。通过真菌发酵利用大豆乳清在乳清处理和高蛋白含量产品的生产方面可能具有经济价值。
