Institute of Microbial Biotechnology, Agricultural University of Georgia, 0131 Tbilisi, Georgia.
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia; Institute of Microbial Biotechnology, Agricultural University of Georgia, 0131 Tbilisi, Georgia.
Int J Med Mushrooms. 2020;22(11):1099-1108. doi: 10.1615/IntJMedMushrooms.2020035655.
The impact of five mushroom inoculum form, age, size, and precultivation medium on the lignocellulose-deconstracting enzyme (LCDE) production was evaluated in the submerged fermentation of mandarin marc. The results obtained evidence that an adaptation of individual fungi to lignocellulose during maintenance in culture collection and inoculum cultivation may be useful for the production of individual LCDE. Homogenization of submerged mycelium was beneficial for all LCDE production by Cerrena unicolor 305 and Ganoderna lucidum 447 and for LME secretion by Coriolopsis gallica 142 and Trametes multicolor 511. Finely chopped mycelial agar favored CMCase and xylanase production by T. multicolor 511 and LiP secretion by C. unicolor 305 and G. lucidum 447 while homogenized mycelial agar proved to be the worst form of inoculum for the production of most enzymes. Four-days inoculum was the most appropriate for the laccase and MnP production by G. lucidum 447 and T. multicolor 511 while the 7-days mycelium provided the highest yields of these enzymes in the cultivation of C. unicolor 305. Use of the 12-days homogenized mycelium from the late stationary phase resulted in lowest laccase activity of all fungi but provided the highest cellulase activity. Overall, the study showed that the LCDE activity and their accumulation profiles in the cultures with different inoculum size was species dependent.
评估了五种蘑菇接种体形式、年龄、大小和预培养介质对柑橘皮渣固态发酵中木质纤维素分解酶（LCDE）生产的影响。结果表明，个体真菌在培养物收集和接种体培养过程中对木质纤维素的适应可能有助于个体 LCDE 的生产。固态发酵菌丝体的均化有利于杂色栓菌 305 和灵芝 447 所有 LCDE 的生产，以及云芝 142 和彩绒革盖菌 511 的 LME 分泌。切碎的菌丝体琼脂有利于彩绒革盖菌 511 的羧甲基纤维素酶和木聚糖酶生产，以及杂色栓菌 305 和灵芝 447 的 LiP 分泌，而均化的菌丝体琼脂被证明是大多数酶生产中最不适宜的接种体形式。4 天的接种体最适合灵芝 447 和彩绒革盖菌 511 的漆酶和 MnP 生产，而 7 天的菌丝体在杂色栓菌 305 的培养中提供了这些酶的最高产量。使用来自晚期静止期的 12 天均化的菌丝体，所有真菌的漆酶活性最低，但提供了最高的纤维素酶活性。总的来说，该研究表明，不同接种体大小的培养物中 LCDE 的活性及其积累谱与物种有关。
