BERRY S J, KRISHNAKUMARAN A, SCHNEIDERMAN H A
Science. 1964 Nov 13;146(3646):938-40. doi: 10.1126/science.146.3646.938.
Injury to diapausing pupae of the cecropia silkworm moth stimulates the synthesis of RNA in all pupal tissues and the synthesis of several blood proteins; such injury also induces the precocious synthesis of a protein, termed "injury protein," which normally appears in the blood during adult development. Actinomycin D, injected in concentrations of 2 micrograms per gram of body weight, blocks the injury-stimulated increase in blood protein synthesis and the injury-induced synthesis of injury protein. However, at concentrations of 0.5 micrograms per gram it prevents the induction of injury-protein synthesis but does not prevent the increased synthesis of other blood proteins. These results suggest that low concentrations of actinomycin may inhibit the synthesis of new kinds of messenger RNA but still permit the continued synthesis of messenger RNA's already in production at the time the actinomycin is injected.
对天蚕蛾滞育蛹的损伤会刺激所有蛹组织中RNA的合成以及几种血蛋白的合成；这种损伤还会诱导一种名为“损伤蛋白”的蛋白质早熟合成，该蛋白通常在成虫发育过程中出现在血液中。以每克体重2微克的浓度注射放线菌素D，会阻断损伤刺激引起的血蛋白合成增加以及损伤诱导的损伤蛋白合成。然而，在每克体重0.5微克的浓度下，它会阻止损伤蛋白的合成诱导，但不会阻止其他血蛋白合成的增加。这些结果表明，低浓度的放线菌素可能会抑制新型信使RNA的合成，但仍允许在注射放线菌素时已经在产生的信使RNA继续合成。
