Hamada T, Setoyama M, Katahira Y, Furuno T, Fujiyoshi T, Sonoda S, Tashiro M
Department of Dermatology, Faculty of Medicine, Kagoshima University, Japan.
J Dermatol Sci. 1992 Sep;4(2):76-82. doi: 10.1016/0923-1811(92)90062-g.
We examined HTLV-I integration patterns in nine cases of HTLV-I-seropositive patients with cutaneous lymphoproliferative disorders. The Southern blot on EcoRI digests of DNA revealed a discrete band of HTLV-I provirus (monoclonal integration) in either skin lesions or peripheral blood lymphocytes (PBL). Four cases showed the monoclonal integration of HTLV-I provirus only in skin lesions: one case showed only in PBL and two cases showed in both skin and PBL. The Southern blot on PstI digests of DNA revealed a 2.4 Kb band of the internal construct of HTLV-I provirus (polyclonal integration) in the PBL of EcoRI-negative samples. The difference in HTLV-I integration patterns between skin lesions and PBL in these cases suggests that the monoclonal outgrowth of HTLV-I-infected cells in the skin is causatively associated with the pathogenesis of cutaneous ATL.
我们检测了9例患有皮肤淋巴增殖性疾病的HTLV-I血清阳性患者的HTLV-I整合模式。DNA经EcoRI酶切后的Southern印迹显示，在皮肤病变或外周血淋巴细胞（PBL）中出现一条离散的HTLV-I前病毒条带（单克隆整合）。4例患者仅在皮肤病变中出现HTLV-I前病毒的单克隆整合：1例仅在PBL中出现，2例在皮肤和PBL中均出现。DNA经PstI酶切后的Southern印迹显示，在EcoRI阴性样本的PBL中出现一条2.4 Kb的HTLV-I前病毒内部构建体条带（多克隆整合）。这些病例中皮肤病变和PBL之间HTLV-I整合模式的差异表明，皮肤中HTLV-I感染细胞的单克隆增殖与皮肤型成人T细胞白血病的发病机制存在因果关系。
