WILLIAMS J, PAYNE W J
Appl Microbiol. 1964 Jul;12(4):360-2. doi: 10.1128/am.12.4.360-362.1964.
Alkyl sulfatase was induced by growth on nutrient broth plus sodium dodecyl sulfate (SDS) in a bacterium we have designated Pseudomonas C12B. Measurement of the radioactivity of S(35)O(4) (=) released from SDS(35) by the enzyme in cell-free extracts provided an effective assay technique. The barium chloranilate assay for release of SO(4) (=) from SDS was somewhat less sensitive but effective if analyzed at 332 mmu. This test was only approximately 55% as sensitive if analyzed at 530 mmu. The activity of the glyoxylate bypass enzymes, isocitrate lyase and malate synthetase, was significantly stimulated by growth of the bacteria on SDS as the sole carbon source, but not by growth on nutrient broth or nutrient broth plus SDS.
在我们命名为假单胞菌C12B的一种细菌中，在营养肉汤加十二烷基硫酸钠（SDS）的培养基上生长可诱导烷基硫酸酯酶的产生。通过测定无细胞提取物中该酶从SDS（35）释放出的S（35）O（4）（=）的放射性，提供了一种有效的检测技术。用氯冉酸钡检测法测定从SDS释放出的SO（4）（=），灵敏度稍低，但在332毫微米处分析时是有效的。如果在530毫微米处分析，该检测的灵敏度仅约为前者的55%。当细菌以SDS作为唯一碳源生长时，乙醛酸循环旁路酶异柠檬酸裂解酶和苹果酸合成酶的活性受到显著刺激，但在营养肉汤或营养肉汤加SDS上生长时则未受刺激。
