Dodgson K S, Fitzgerald J W, Payne W J
Biochem J. 1974 Jan;138(1):53-62. doi: 10.1042/bj1380053.
When Pseudomonas C12B is grown on nutrient broth to the stationary phase, cell extracts contain two secondary alkylsulphatases (S1 and S2) active towards potassium decan-5-yl sulphate but not towards potassium pentan-3-yl sulphate and one primary alkylsulphatase (P1) active towards sodium dodecan-1-yl sulphate (sodium dodecyl sulphate). When 10mm-sodium hexan-1-yl sulphate is included in the nutrient broth an additional primary alkylsulphatase (P2) is produced. The S1, S2, P1 and P2 enzymes are also present in extracts of cells grown on broth containing the commercial detergent Oronite, together with an additional secondary alkylsulphatase (S3) active towards pentan-3-yl sulphate as well as decan-5-yl sulphate. The P2 primary alkylsulphatase can be induced by a number of primary and secondary alkyl sulphate esters but the induction of the S3 enzyme appears to be a more specific and complex process. Studies on the ability of different fractions separated from Oronite to act as inducers suggest that the combination of a long-chain secondary alkyl sulphate(s) and a long-chain secondary alcohol(s) is responsible for the appearance of the S3 enzyme. Potassium hexadecan-2-yl sulphate or potassium tetradecan-2-yl sulphate, in combination with either hexadecan-2-ol or tetradecan-2-ol, can serve as inducers for the enzyme. Some characteristics of these specific inducer systems have been elucidated.
当铜绿假单胞菌C12B在营养肉汤中生长至稳定期时，细胞提取物中含有两种对癸烷-5-基硫酸钾有活性但对戊烷-3-基硫酸钾无活性的仲烷基硫酸酯酶（S1和S2），以及一种对十二烷-1-基硫酸钠（十二烷基硫酸钠）有活性的伯烷基硫酸酯酶（P1）。当在营养肉汤中加入10 mM己烷-1-基硫酸钠时，会产生另一种伯烷基硫酸酯酶（P2）。S1、S2、P1和P2酶也存在于在含有商业洗涤剂奥罗尼特的肉汤中生长的细胞提取物中，同时还存在另一种对戊烷-3-基硫酸酯以及癸烷-5-基硫酸酯有活性的仲烷基硫酸酯酶（S3）。P2伯烷基硫酸酯酶可被多种伯烷基和仲烷基硫酸酯诱导，但S3酶的诱导似乎是一个更具特异性和复杂性的过程。对从奥罗尼特中分离出的不同组分作为诱导剂的能力进行的研究表明，长链仲烷基硫酸酯和长链仲醇的组合是S3酶出现的原因。十六烷-2-基硫酸钾或十四烷-2-基硫酸钾与十六烷-2-醇或十四烷-2-醇组合，可作为该酶的诱导剂。这些特定诱导剂系统的一些特性已得到阐明。
