Hanker J S, Kasler F, Bloom M G, Copeland J S, Seligman A M
Science. 1967 Jun 30;156(3783):1737-8. doi: 10.1126/science.156.3783.1737.
The design of cytochemical reagents that yield osmiophilic products from which an osmium black may be derived on exposure to osmium tetroxide has resulted in new methods described previously for the ultrastructural demonstration of enzyme activity and functional groups of macromolecules with the electron microscope. Attempts to determine the nature of the osmium black end products have been frustrated by their insolubility. The preparation of watersoluble analogs and their characterization as polymers suggest that the insoluble osmium blacks are coordination polymers. This is consonant with the unusually favorable properties of osmium black in electron microscopy. Although polymers of osmium have frequently been postulated as the end products of reaction of osmium tetroxide with tissue conistituents or with other organic compounds, this is the first example of their characterization.
细胞化学试剂的设计可产生嗜锇产物，在暴露于四氧化锇时可从中衍生出锇黑，这导致了先前描述的用电子显微镜超微结构显示酶活性和大分子官能团的新方法。由于锇黑最终产物不溶，确定其性质的尝试受到了阻碍。水溶性类似物的制备及其作为聚合物的表征表明，不溶性锇黑是配位聚合物。这与锇黑在电子显微镜中异常良好的特性相一致。尽管锇聚合物经常被假定为四氧化锇与组织成分或其他有机化合物反应的最终产物，但这是对其进行表征的第一个例子。
