Hanker J S, Anderson W A, Bloom F E
Science. 1972 Mar 3;175(4025):991-3. doi: 10.1126/science.175.4025.991.
A transition metal compound that is bound in tissues by any appropriate cytochemical reaction may catalyze the generation of an insoluble osmiophilic polymer from organic monomers such as 3,3'-diaminobenzidine. When the polymers are treated with osmium tetroxide, electron-opaque, insoluble osmium blacks (coordination polymers of osmium) are formed at the sites of the particular macromolecule or enzyme permitting its light, and electron, microscopic localization. This approach represents a distinct advantage over earlier cytochemical methods because the shorter incubation time needed here results in less artifactual deposition of metal ions, and less tendency to crystallize the reaction product. In addition, the shorter incubation times permit longer fixation of tissues and hence less artifact due to enzyme diffusion.
通过任何适当的细胞化学反应结合在组织中的过渡金属化合物可以催化由有机单体（如3,3'-二氨基联苯胺）生成不溶性嗜锇聚合物。当用四氧化锇处理这些聚合物时，在特定大分子或酶的部位会形成电子不透明的不溶性锇黑（锇的配位聚合物），从而可以通过光学显微镜和电子显微镜对其进行定位。这种方法相对于早期的细胞化学方法具有明显的优势，因为这里所需的孵育时间较短，导致金属离子的人为沉积较少，并且反应产物结晶的倾向较小。此外，较短的孵育时间允许对组织进行更长时间的固定，因此由于酶扩散导致的假象较少。
