YIP L C, SHAH R, DAY R A
J Bacteriol. 1964 Aug;88(2):297-308. doi: 10.1128/jb.88.2.297-308.1964.
Yip, Lily C. (University of Cincinnati, Cincinnati, Ohio), Ramesh Shah, and Richard A. Day. Metabolic control of penicillinase biosynthesis in Bacillus cereus. J. Bacteriol. 88:297-308. 1964.-Penicillinase production in strains 5 and 5/B of Bacillus cereus in response to treatment by 6-aminopenicillanic acid (APA), penicillin G, (6-N-alpha-(p-benzyloxyphenoxy)-propionylamino-penicillanic acid, and cephalosporin C (CC) was found to be analogous to that seen in constitutive strains. Strain 5 did not release penicillinase into the medium to any great extent. Penicillinase production and the effect of the above penicillins on it were found to decline with increasing density of the culture. The penicillins were shown to accelerate or retard the production of penicillinase activity in strain 5 cells during pretreatment at 0 C and during incubation at 37 C. Strains 5 and 5/B gave qualitatively similar responses to penicillin treatment. At 0 C, the specific activity of penicillinase in strain 5 passes through a period of rapid increase at 0 hr and a period of little change at approximately 1 hr, followed by an increased rate of change towards 2 hr. The effect of APA or CC on specific activity of strain 5 cells during treatment at 0 C could not be reversed by one another, but Hg could reverse the increase caused by CC to some extent and the repression caused by APA. The production of penicillinase in the microconstitutive strain 5 of Bacillus cereus in response to treatment with CC was influenced by various inhibitors. 8-Azaguanine inhibited the production of the enzyme both during a pretreatment of the cells with CC at 0 C and during the subsequent incubation at 37 C. Actinomycin D, 6-azauracil, 6-thioguanine, and 2-thiocytosine inhibit the increase in penicillinase arising after the pretreatment at 0 C. 6-Azathymine has very little effect on the change of penicillinase activity. The CC-induced change occurring during the 0 C period was postulated to be a process at the level of protein biosynthesis itself; change at 37 C, constituting a delayed response, was considered a process at the level of messenger ribonucleic acid synthesis.
叶，莉莉·C.（辛辛那提大学，俄亥俄州辛辛那提），拉梅什·沙阿，和理查德·A.戴。蜡样芽孢杆菌中青霉素酶生物合成的代谢控制。《细菌学杂志》88:297 - 308。1964年。- 发现蜡样芽孢杆菌5型和5/B型菌株在受到6 - 氨基青霉烷酸（APA）、青霉素G、（6 - N - α -（对苄氧基苯氧基）- 丙酰氨基青霉烷酸）和头孢菌素C（CC）处理时产生青霉素酶的情况与组成型菌株相似。5型菌株在很大程度上不会将青霉素酶释放到培养基中。随着培养物密度的增加，青霉素酶的产生以及上述青霉素对其的影响均下降。在0℃预处理期间和37℃培养期间，青霉素被证明可加速或延缓5型菌株细胞中青霉素酶活性的产生。5型和5/B型菌株对青霉素处理的定性反应相似。在0℃时，5型菌株中青霉素酶的比活性在0小时经历快速增加期，在大约1小时经历变化很小的时期，随后在接近2小时时变化速率增加。在0℃处理期间，APA或CC对5型菌株细胞比活性的影响不能相互逆转，但汞可在一定程度上逆转CC引起的增加和APA引起的抑制。蜡样芽孢杆菌微组成型5型菌株在受到CC处理时青霉素酶的产生受到各种抑制剂的影响。在0℃用CC对细胞进行预处理期间以及随后在37℃培养期间，8 - 氮杂鸟嘌呤均抑制该酶的产生。放线菌素D、6 - 氮尿嘧啶、6 - 硫鸟嘌呤和2 - 硫胞嘧啶抑制0℃预处理后青霉素酶的增加。6 - 氮胸腺嘧啶对青霉素酶活性的变化影响很小。在0℃期间CC诱导的变化被假定为蛋白质生物合成水平本身的一个过程；在37℃的变化构成延迟反应，被认为是信使核糖核酸合成水平的一个过程。
