Pegg A E, Stanley B A, Wiest L, Casero R A
Department of Cellular and Molecular Physiology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033.
Biochim Biophys Acta. 1992 Nov 15;1171(1):106-8. doi: 10.1016/0167-4781(92)90148-s.
The nucleotide sequence of a cDNA encoding hamster spermidine/spermine-N1-acetyltransferase, a key enzyme in polyamine degradation and excretion, has been determined. The cDNA consists of a 1016 base pair insert including 120 nucleotides of the 5' untranslated region and the complete 3' untranslated region. The deduced amino acid sequence is very similar to the human spermidine/spermine-N1-acetyltransferase with only 8 differences in 171 amino acids and the corresponding nucleotide sequence shows 91% identity. The 5' untranslated regions are even more closely related with 97% identity suggesting that this region may play a role in the regulation of acetyltransferase activity. Translation of the acetyltransferase mRNA in a reticulocyte lysate was not altered by the addition of N1,N12-bis(ethyl)spermine.
已确定编码仓鼠亚精胺/精胺 - N1 - 乙酰转移酶（多胺降解和排泄中的关键酶）的cDNA的核苷酸序列。该cDNA由一个1016个碱基对的插入片段组成，包括5'非翻译区的120个核苷酸和完整的3'非翻译区。推导的氨基酸序列与人类亚精胺/精胺 - N1 - 乙酰转移酶非常相似，在171个氨基酸中只有8个差异，相应的核苷酸序列显示出91%的同一性。5'非翻译区的相关性更强，同一性为97%，这表明该区域可能在乙酰转移酶活性的调节中起作用。在网织红细胞裂解物中添加N1,N12 - 双（乙基）精胺不会改变乙酰转移酶mRNA的翻译。
