Xiao L, Celano P, Mank A R, Pegg A E, Casero R A
Oncology Center Laboratories, Johns Hopkins University School of Medicine, Baltimore, MD 21231.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):407-15. doi: 10.1016/0006-291x(91)91385-p.
Spermidine/spermine N1-acetyltransferase is the rate-limiting enzyme in the catabolism of cellular polyamines. Using a combination of cDNA library screening and anchored PCR methodologies, a full length cDNA designated AP3/F7 corresponding to the human SSAT was cloned using RNA from the human large cell undifferentiated lung carcinoma line NCI H157. The resulting cDNA clone is 1,060 base pairs with a 513 base open reading frame coding for a 171 amino acid protein, with a predicted subunit molecular weight of 20,023. The 5' non-coding region of AP3/F7 is 165 bases and the 3' untranslated region is 382 bases with a polyadenylation site 20 bases 5' to the poly(A) tail. This full length cDNA should be an aid in the study of the regulation of spermidine/spermine N1-acetyltransferase expression and the significance of the acetyltransferase in polyamine metabolism.
亚精胺/精胺N1 - 乙酰基转移酶是细胞多胺分解代谢中的限速酶。通过结合cDNA文库筛选和锚定PCR方法，使用来自人类大细胞未分化肺癌细胞系NCI H157的RNA克隆了一个对应于人精胺/亚精胺N1 - 乙酰基转移酶（SSAT）的全长cDNA，命名为AP3/F7。得到的cDNA克隆为1060个碱基对，具有一个513个碱基的开放阅读框，编码一个171个氨基酸的蛋白质，预测亚基分子量为20,023。AP3/F7的5'非编码区为165个碱基，3'非翻译区为382个碱基，在多聚腺苷酸尾巴5'端20个碱基处有一个多聚腺苷酸化位点。这个全长cDNA应该有助于研究亚精胺/精胺N1 - 乙酰基转移酶表达的调控以及该乙酰基转移酶在多胺代谢中的意义。
