Pendyala L, Wellman A M
J Bacteriol. 1977 Aug;131(2):453-62. doi: 10.1128/jb.131.2.453-462.1977.
Although germinated conidia of Neurospora crassa transport adenine through two different systems, only one of these, namely, the general purine transport system, which transports adenine, hypoxanthine, guanine, and 6-methylpurine, is present in freshly harvested conidia of the wild type. The second system develops during germination. The latter system can transport adenine and 6-methylpurine. Time course and kinetic studies of adenine transport in freshly harvested conidia of an ad-8 mutant indicated that, in contrast to the wild type, the general purine transport activity is very low in this strain and that the second adenine transport system is possibly present in the ungerminated conidia. A study of adenine and hypoxanthine uptake in ad-8 and ad-4 mutants, both of which cannot utilize hypoxanthine for growth, isolated that the two transport systems may be under different metabolic controls.
尽管粗糙脉孢菌的萌发分生孢子通过两种不同的系统转运腺嘌呤，但野生型刚收获的分生孢子中仅存在其中一种，即通用嘌呤转运系统，该系统可转运腺嘌呤、次黄嘌呤、鸟嘌呤和6-甲基嘌呤。第二种系统在萌发过程中发育。后一种系统可以转运腺嘌呤和6-甲基嘌呤。对ad-8突变体刚收获的分生孢子中腺嘌呤转运的时间进程和动力学研究表明，与野生型相比，该菌株中的通用嘌呤转运活性非常低，并且第二种腺嘌呤转运系统可能存在于未萌发的分生孢子中。对ad-8和ad-4突变体中腺嘌呤和次黄嘌呤摄取的研究（这两种突变体均不能利用次黄嘌呤进行生长）表明，这两种转运系统可能受不同的代谢控制。
