嗜硫硫杆菌中某些三羧酸循环酶存在的证据。
EVIDENCE FOR THE PRESENCE OF CERTAIN TRICARBOXYLIC ACID CYCLE ENZYMES IN THIOBACILLUS THIOPARUS.
作者信息
COOPER R C
出版信息
J Bacteriol. 1964 Sep;88(3):624-9. doi: 10.1128/jb.88.3.624-629.1964.
Cooper, Robert C. (Michigan State University, East Lansing). Evidence for the presence of certain tricarboxylic acid cycle enzymes in Thiobacillus thioparus. J. Bacteriol. 88:624-629. 1964.-Various tricarboxylic acid cycle enzymes appear to be present in Thiobacillus thioparus. Cell-free extracts of T. thioparus were active for a number of tricarboxylic acid cycle enzymes, including aconitase, isocitric dehydrogenase, and malic dehydrogenase. Tests for the presence of fumarase and the condensing enzyme, citrogenase, were inconclusive. Citrate was shown to be active in the metabolism of T. thioparus, but the actual mechanism involved in its formation was not clear. The enzyme, isocitratase, appeared to be absent. Evidence for the presence of succinic dehydrogenase was found in experiments with whole cells. From these results, it would appear that T. thioparus has a terminal respiration pathway similar to that found in many heterotrophic microorganisms.
库珀，罗伯特·C.（密歇根州立大学，东兰辛）。关于排硫硫杆菌中某些三羧酸循环酶存在的证据。《细菌学杂志》88:624 - 629。1964年。——排硫硫杆菌中似乎存在多种三羧酸循环酶。排硫硫杆菌的无细胞提取物对多种三羧酸循环酶有活性，包括乌头酸酶、异柠檬酸脱氢酶和苹果酸脱氢酶。对延胡索酸酶和缩合酶（柠檬酸合酶）存在的检测结果尚无定论。已证明柠檬酸盐在排硫硫杆菌的代谢中具有活性，但其形成所涉及的实际机制尚不清楚。异柠檬酸裂合酶似乎不存在。在对全细胞的实验中发现了琥珀酸脱氢酶存在的证据。从这些结果来看，排硫硫杆菌似乎具有与许多异养微生物中发现的类似的末端呼吸途径。
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