VANDEMARK P J, SMITH P F
J Bacteriol. 1964 Dec;88(6):1602-7. doi: 10.1128/jb.88.6.1602-1607.1964.
VanDemark, P. J. (Cornell University, Ithaca, N.Y.), and P. F. Smith. Evidence for a tricarboxylic acid cycle in Mycoplasma hominis. J. Bacteriol. 88:1602-1607. 1964.-Resting cells of acetate-grown Mycoplasma hominis strain 07 oxidized the various intermediates of the tricarboxylic and glyoxylate cycles, with the exception of sodium citrate and glyoxylate. Extracts of these cells possessed isocitric dehydrogenase, isocitratase, alpha-ketoglutaric dehydrogenase, succinic dehydrogenase, fumarase, malic dehydrogenase, citratase, and acetyl coenzyme A kinase activities. With the assay conditions employed, condensing enzyme, malate synthetase, and phosphotransacetylase activities were negligible. Incubation of sodium acetate-2-C(14) with the various intermediates of the tricarboxylic acid cycle in the presence of cell-free extracts resulted in exchange of the isotope with these compounds as well as the formation of other labeled intermediates of the tricarboxylic acid cycle. Oxidation of sodium acetate-2-C(14) alone resulted in the formation of labeled succinate, fumarate, and malate.
范德马克，P. J.（纽约州伊萨卡市康奈尔大学）和P. F. 史密斯。人型支原体中三羧酸循环的证据。《细菌学杂志》88:1602 - 1607。1964年。——以乙酸盐培养的人型支原体07菌株的静息细胞氧化三羧酸循环和乙醛酸循环的各种中间产物，但柠檬酸钠和乙醛酸除外。这些细胞的提取物具有异柠檬酸脱氢酶、异柠檬酸裂合酶、α - 酮戊二酸脱氢酶、琥珀酸脱氢酶、延胡索酸酶、苹果酸脱氢酶、柠檬酸合成酶和乙酰辅酶A激酶活性。在所采用的测定条件下，缩合酶、苹果酸合成酶和磷酸转乙酰酶的活性可忽略不计。在无细胞提取物存在的情况下，将乙酸钠 - 2 - C(14)与三羧酸循环的各种中间产物一起温育，导致同位素与这些化合物交换，以及形成三羧酸循环的其他标记中间产物。单独氧化乙酸钠 - 2 - C(14)导致形成标记的琥珀酸、延胡索酸和苹果酸。
