Härtig W, Brauer K, Brückner G
Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
Neuroreport. 1992 Oct;3(10):869-72. doi: 10.1097/00001756-199210000-00012.
Net-like structures surrounding several types of neurones contain glycoconjugates which are detectable by lectins specific for N-acetylgalactosamine. Wisteria floribunda agglutinin (WFA) was introduced as a further marker for the visualization of such perineuronal nets, which were also revealed in regions of the rat brain where these structures could not be clearly demonstrated using other lectins. The WFA-labelled perineuronal nets resembled in detail those which could be visualized using Vicia villosa agglutinin, colloidal iron or hyaluronectin as markers. Furthermore, WFA-stained perineuronal net components appeared to be similar to proteoglycan-immunoreactive structures. Dual-peroxidase experiments and fluorescence double labelling demonstrated that WFA-binding structures frequently ensheath GABAergic neurons containing the calcium-binding protein parvalbumin in the areas investigated.
围绕几种类型神经元的网状结构含有糖缀合物，这些糖缀合物可被对N - 乙酰半乳糖胺具有特异性的凝集素检测到。紫藤凝集素（WFA）被用作可视化此类神经元周围网的进一步标记物，在大鼠脑的一些区域也发现了这些结构，而使用其他凝集素无法清晰显示这些结构。WFA标记的神经元周围网在细节上类似于使用绒毛豌豆凝集素、胶体铁或透明质酸结合蛋白作为标记物可视化的那些结构。此外，WFA染色的神经元周围网成分似乎与蛋白聚糖免疫反应性结构相似。双过氧化物酶实验和荧光双重标记表明，在所研究的区域中，WFA结合结构经常包裹着含有钙结合蛋白小白蛋白的GABA能神经元。
