蛋白水解酶对大肠杆菌噬菌体及天然蛋白质的作用
THE EFFECT OF PROTEOLYTIC ENZYMES ON E. COLI PHAGES AND ON NATIVE PROTEINS.
作者信息
NORTHROP J H
出版信息
J Gen Physiol. 1964 Sep;48(1):73-8. doi: 10.1085/jgp.48.1.73.
Lambda coli phage is not inactivated by chymotrypsin, trypsin, or ficin. T(2) phage is slowly inactivated by high concentrations of (alpha-, beta-, gamma-, or Delta-chymotrypsin, but not by trypsin or ficin. P(1) phage is slowly inactivated by alpha-, beta-, or gamma-chymotrypsin, or ficin, more rapidly by Delta-chymotrypsin, and much more rapidly by trypsin. Crystalline egg albumin, crystalline serum albumin, E. coli nucleoprotein, and yeast nucleoprotein are hydrolyzed slowly by alpha-chymotrypsin. Yeast nucleoprotein, like P(1) phage, is hydrolyzed more rapidly by Delta-chymotrypsin than by alpha-chymotrypsin, but not by trypsin or ficin. Neither phages nor native proteins were attacked by papain, carboxypeptidase, deoxyribonuclease, or ribonuclease.
λ 型大肠杆菌噬菌体不会被胰凝乳蛋白酶、胰蛋白酶或无花果蛋白酶灭活。T(2) 噬菌体在高浓度的（α-、β-、γ- 或 δ- 胰凝乳蛋白酶作用下会缓慢灭活，但不会被胰蛋白酶或无花果蛋白酶灭活。P(1) 噬菌体在 α-、β- 或 γ- 胰凝乳蛋白酶或无花果蛋白酶作用下会缓慢灭活，在 δ- 胰凝乳蛋白酶作用下灭活速度更快，而在胰蛋白酶作用下灭活速度则快得多。结晶卵清蛋白、结晶血清白蛋白、大肠杆菌核蛋白和酵母核蛋白在 α- 胰凝乳蛋白酶作用下会被缓慢水解。酵母核蛋白与 P(1) 噬菌体一样，在 δ- 胰凝乳蛋白酶作用下比在 α- 胰凝乳蛋白酶作用下被水解得更快，但不会被胰蛋白酶或无花果蛋白酶水解。无论是噬菌体还是天然蛋白质都不会被木瓜蛋白酶、羧肽酶、脱氧核糖核酸酶或核糖核酸酶攻击。
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