Drowart A, Selleslaghs J, Yernault J C, Valcke C, De Bruyn J, Huygen K, Farber C M, Van Vooren J P
Université libre de Bruxelles, Belgium.
Tuber Lung Dis. 1992 Jun;73(3):137-40. doi: 10.1016/0962-8479(92)90146-B.
The specific IgG response induced by BCG vaccination was investigated in 27 adults using two purified BCG antigens in a dot immunoblotting assay. The reflectance values increased significantly after vaccination when P64 but not when P32 was used as the antigen. The values measured after vaccination were compared with those obtained when testing sera from patients with tuberculosis. We observed that the level of anti-P64 IgG reached after BCG immunization was high enough to preclude a serological differential diagnosis with tuberculosis.
在27名成年人中，使用两种纯化的卡介苗抗原通过斑点免疫印迹法研究了卡介苗接种诱导的特异性IgG反应。当使用P64作为抗原时，接种疫苗后反射率值显著增加，而使用P32作为抗原时则不然。将接种疫苗后测得的值与检测结核病患者血清时获得的值进行比较。我们观察到，卡介苗免疫后达到的抗P64 IgG水平高到足以排除与结核病的血清学鉴别诊断。
