Krambovitis E
J Med Microbiol. 1986 May;21(3):257-64. doi: 10.1099/00222615-21-3-257.
Antibody activity against Mycobacterium tuberculosis of sera from an area with a high prevalence of tuberculosis was measured by enzyme-linked immunosorbent assay (ELISA) with a plasma-membrane extract from M. tuberculosis strain H37RV. All sera from relapsed tuberculosis patients and 82.5% of sera from new untreated cases gave positive results. The seronegative group of tuberculosis patients gave positive results by direct microscopy and culture. No clear correlation between antibody and delayed hypersensitivity or extent of disease was observed. Chemotherapy was associated with a higher antibody response. Specificity of the test with healthy control subjects from the high prevalence area was 85%. Negative results were obtained with 145 sera from presumed healthy European subjects and with seven sera from BCG-vaccinated subjects.
采用酶联免疫吸附测定（ELISA），以结核分枝杆菌H37RV株的质膜提取物为抗原，检测结核病高流行地区血清针对结核分枝杆菌的抗体活性。所有复发结核病患者的血清以及82.5%新诊断未治疗病例的血清检测结果均为阳性。结核病血清学阴性组患者经直接显微镜检查和培养显示阳性结果。未观察到抗体与迟发型超敏反应或疾病严重程度之间存在明显相关性。化疗与更高的抗体反应相关。该检测在结核病高流行地区健康对照人群中的特异性为85%。145份来自假定健康的欧洲受试者的血清以及7份来自接种卡介苗受试者的血清检测结果均为阴性。
