Bensinger T A, Chillar R K, Beutler E
J Lab Clin Med. 1977 Mar;89(3):498-503.
A close relationship exists between the decrease in concentration of 2,3-diphosphoglycerate (2,3-DPG) and a fall in the pH of stored blood. Buffering the stored red cells with bicarbonate is one solution to the problem of maintaining pH during storage. The effectiveness of this buffer depends upon loss from the stored blood of carbonic acid in the form of CO2. We describe a system in which the CO2 is trapped in a small internal package which contains calcium hydroxide, or calcium hydroxide embedded in Silastic. A medium containing bicarbonate, adenine, glucose, phosphate and mannitol (BAGPM) is added after initial packing of the erythrocytes. With this approach, it has been possible to maintain 2,3-DPG at 92 percent of original, and ATP was approximately 62 percent of initial levels at the end of 42 days of storage if an internal Silastic bag containing calcium was used in bags agitated once weekly. More frequent agitation (five times weekly) produced acceptable maintenance of both 2,3-DPG (78 percent of original) and ATP (44 percent of original) after 42 days of storage when a Silastic block impregnated with calcium hydroxide was utilized to absorb CO2.
2,3-二磷酸甘油酸（2,3-DPG）浓度降低与储存血液pH值下降之间存在密切关系。用碳酸氢盐缓冲储存的红细胞是解决储存期间维持pH值问题的一种方法。这种缓冲液的有效性取决于以二氧化碳形式从储存血液中损失的碳酸。我们描述了一种系统，其中二氧化碳被困在一个小的内部包装中，该包装含有氢氧化钙或嵌入硅橡胶中的氢氧化钙。在红细胞初步包装后，加入含有碳酸氢盐、腺嘌呤、葡萄糖、磷酸盐和甘露醇（BAGPM）的培养基。采用这种方法，如果在每周搅拌一次的袋子中使用含有钙的内部硅橡胶袋，则在储存42天后可以将2,3-DPG维持在原始水平的92%，ATP约为初始水平的62%。当使用浸渍有氢氧化钙的硅橡胶块吸收二氧化碳时，更频繁的搅拌（每周五次）在储存42天后可使2,3-DPG（原始水平的78%）和ATP（原始水平的44%）得到可接受的维持。
