BURCHALL J J, NIEDERMAN R A, WOLIN M J
J Bacteriol. 1964 Oct;88(4):1038-44. doi: 10.1128/jb.88.4.1038-1044.1964.
Burchall, J. J. (University of Illinois, Urbana), R. A. Niederman, and M. J. Wolin. Amino group formation and glutamate synthesis in Streptococcus bovis. J. Bacteriol. 88:1038-1044. 1964.-Extracts of Streptococcus bovis grown on NH(4) (+) as a nitrogen source contain a nicotinamide adenine dinucleotide phosphate (NADP)-linked glutamic dehydrogenase and are devoid of alanine dehydrogenase, other amino acid dehydrohygenases, and aspartase. A potential source of reduced nicotinamide adenine dinucleotide phosphate for glutamate synthesis is a NADP and nicotinamide adenine dinucleotide (NAD)-linked glyceraldehyde-3-phosphate dehydrogenase present in the extracts. Experiments with C(14)-labeled glucose and NaHCO(3) indicate that the glutamate carbon skeleton is synthesized by a tricarboxylic acid pathway. The synthesis of the carbon skeleton of glutamate is repressed when glutamate or casein hydrolysate supplement the NH(4) (+)-containing growth medium. Repression of glutamic dehydrogenase and a NAD-linked isocitric dehydrogenase occurs only when complex nitrogen sources, but not when free amino acids, are added to the growth medium.
伯查尔，J. J.（伊利诺伊大学厄巴纳分校）、R. A. 尼德曼和M. J. 沃林。牛链球菌中氨基的形成和谷氨酸的合成。《细菌学杂志》88:1038 - 1044。1964年。——以NH₄⁺作为氮源生长的牛链球菌提取物含有一种烟酰胺腺嘌呤二核苷酸磷酸（NADP）连接的谷氨酸脱氢酶，并且缺乏丙氨酸脱氢酶、其他氨基酸脱氢酶和天冬氨酸酶。提取物中存在的一种NADP和烟酰胺腺嘌呤二核苷酸（NAD）连接的3 - 磷酸甘油醛脱氢酶是谷氨酸合成中还原型烟酰胺腺嘌呤二核苷酸磷酸的一个潜在来源。用¹⁴C标记的葡萄糖和NaHCO₃进行的实验表明，谷氨酸的碳骨架是通过三羧酸途径合成的。当谷氨酸或酪蛋白水解物补充含NH₄⁺的生长培养基时，谷氨酸碳骨架的合成受到抑制。仅当向生长培养基中添加复合氮源而非游离氨基酸时，谷氨酸脱氢酶和一种NAD连接的异柠檬酸脱氢酶才会受到抑制。
