Dennen D W, Niederpruem D J
J Bacteriol. 1967 Mar;93(3):904-13. doi: 10.1128/jb.93.3.904-913.1967.
The specific activities of two glutamate dehydrogenases (GDH), one requiring nicotinamide adenine dinucleotide (NAD) and the other specific for nicotinamide adenine dinucleotide phosphate (NADP), varied during growth of Schizophyllum commune as a function of the stage of the life cycle and the exogenous nitrogen source. During basidiospore germination on either glucose-NH(3) or glucose-glutamate medium, NADP-GDH increased six- to eightfold in specific activity, whereas NAD-GDH was depressed. During dikaryotic mycelial growth on either nitrogen source, the two GDH increased in a 1:1 ratio, whereas, during homokaryotic mycelial growth on glucose-NH(3), NADP-GDH activity was depressed and NAD-GDH increased six- to eightfold. Homokaryotic mycelium cultured on glucose-glutamate medium yielded high NADP-GDH activities and normal NAD-GDH activities. Intracellular NH(3) concentration and NADP-GDH activities were inversely related during spore germination and homokaryotic mycelium growth, whereas guanosine-5'-triphosphate (GTP) and l-glutamine specifically inhibited NAD- and NADP-GDH respectively in vitro. GTP inhibition was shown in extracts from cells at all stages of the life cycle. Basidiospore germling extracts contained an NADP-GDH essentially resistant to l-glutamine inhibition.
两种谷氨酸脱氢酶（GDH）的比活性，一种需要烟酰胺腺嘌呤二核苷酸（NAD），另一种对烟酰胺腺嘌呤二核苷酸磷酸（NADP）具有特异性，在裂褶菌生长过程中随生命周期阶段和外源氮源而变化。在葡萄糖 - NH₃或葡萄糖 - 谷氨酸培养基上担孢子萌发期间，NADP - GDH的比活性增加了六至八倍，而NAD - GDH则受到抑制。在任何一种氮源上双核菌丝体生长期间，两种GDH以1:1的比例增加，而在葡萄糖 - NH₃上单核菌丝体生长期间，NADP - GDH活性受到抑制，NAD - GDH增加了六至八倍。在葡萄糖 - 谷氨酸培养基上培养的单核菌丝体产生高NADP - GDH活性和正常的NAD - GDH活性。在孢子萌发和单核菌丝体生长期间，细胞内NH₃浓度与NADP - GDH活性呈负相关，而鸟苷 - 5'-三磷酸（GTP）和L - 谷氨酰胺分别在体外特异性抑制NAD - GDH和NADP - GDH。在生命周期的所有阶段的细胞提取物中均显示出GTP抑制作用。担孢子萌发提取物中含有一种基本上对L - 谷氨酰胺抑制有抗性的NADP - GDH。
