Kobayashi T, Robertson B, Grossmann G, Nitta K, Curstedt T, Suzuki Y
Department of Anesthesiology, School of Medicine, Kanazawa University, Japan.
Acta Paediatr. 1992 Sep;81(9):665-71. doi: 10.1111/j.1651-2227.1992.tb12329.x.
A monoclonal antibody to the surfactant-associated hydrophobic protein SP-B was added at various concentrations to a standard preparation of porcine surfactant (Curosurf 10 mg/ml), and surface properties were evaluated with pulsating bubble. Retarded adsorption of surfactant was observed at antibody concentrations > or = 0.5 mg/ml and significantly increased values for minimum surface tension were observed at antibody concentrations > or = 1 mg/ml. In vivo effects of the antibody were tested in immature newborn rabbits ventilated with a standardized sequence of insufflation pressures. Animals receiving 0.1 ml surfactant (80 mg/ml) mixed with antibody at concentrations > or = 4 mg/ml had low tidal volumes, poor lung stability in pressure-volume recordings, poor alveolar expansion in histological sections and widespread epithelial necrosis in peripheral airways. Admixtures of IgG had no such effects. We conclude that this monoclonal antibody inactivates exogenous porcine surfactant, probably by preventing fast adsorption of surfactant lipids to the alveolar air-liquid interfaces.
将针对表面活性剂相关疏水蛋白SP - B的单克隆抗体以不同浓度添加到猪表面活性剂的标准制剂（固尔苏，10毫克/毫升）中，并用脉动气泡法评估表面特性。在抗体浓度≥0.5毫克/毫升时观察到表面活性剂的吸附延迟，在抗体浓度≥1毫克/毫升时观察到最小表面张力值显著增加。在以标准化吹入压力序列通气的未成熟新生兔中测试了该抗体的体内效应。接受0.1毫升表面活性剂（80毫克/毫升）与浓度≥4毫克/毫升的抗体混合液的动物潮气量低，压力 - 容积记录中肺稳定性差，组织学切片中肺泡扩张不良，外周气道广泛上皮坏死。IgG混合物没有此类效应。我们得出结论，这种单克隆抗体可能通过阻止表面活性剂脂质快速吸附到肺泡气 - 液界面而使外源性猪表面活性剂失活。
