WITTENBERGER C L, HAAF A S
J Bacteriol. 1964 Oct;88(4):896-903. doi: 10.1128/jb.88.4.896-903.1964.
Wittenberger, Charles L. (National Institute of Dental Research, U.S. Public Health Service, Bethesda, Md.), and Ann S. Haaf. Lactate-degrading system in Butyribacterium rettgeri subject to glucose repression. J. Bacteriol. 88:896-903. 1964.-The ability of Butyribacterium rettgeri to utilize lactate as the main energy source for growth requires the formation of a lactate-degrading system. The precise nature of this system is unknown, but preliminary evidence suggests that cellular acquisition of lactate-decomposing activity involves the formation of a nonpyridine nucleotide-linked lactic dehydrogenase. This enzyme, which can couple lactate oxidation to the reduction of ferricyanide [K(3)Fe(CN)(6)-lactic de-hydrogenase (LDH)], is absent from glucose-grown cells; this observation appears to account for the inability of such cells to decompose lactate even though they may form lactate from glucose. The formation of K(3)Fe(CN)(6)-LDH in growing cultures requires the addition of lipoic acid to the medium, and is repressed by glucose, pyruvate, or fructose. When any of the latter substrates are included in the growth medium with lactate, nicotinamide adenine dinucleotide-linked LDH activity is present in cells at markedly higher levels than it is in cells grown on lactate alone.
维滕贝格尔，查尔斯·L.（美国国立牙科研究所，美国公共卫生服务部，马里兰州贝塞斯达），以及安·S.哈夫。瑞氏丁酸杆菌中受葡萄糖阻遏的乳酸降解系统。《细菌学杂志》88:896 - 903。1964年。——瑞氏丁酸杆菌利用乳酸作为主要生长能量来源的能力需要形成一个乳酸降解系统。该系统的确切性质尚不清楚，但初步证据表明，细胞获得乳酸分解活性涉及一种非吡啶核苷酸连接的乳酸脱氢酶的形成。这种能将乳酸氧化与铁氰化物还原相偶联的酶[K(3)Fe(CN)(6)-乳酸脱氢酶（LDH）]，在以葡萄糖为生长底物的细胞中不存在；这一观察结果似乎解释了这类细胞即使能从葡萄糖生成乳酸却无法分解乳酸的原因。在生长培养物中K(3)Fe(CN)(6)-LDH的形成需要向培养基中添加硫辛酸，并且会受到葡萄糖、丙酮酸或果糖的阻遏。当在含有乳酸的生长培养基中加入上述任何一种底物时，细胞中烟酰胺腺嘌呤二核苷酸连接的LDH活性水平明显高于仅以乳酸为生长底物的细胞。
